Systems biology for organotypic cell cultures

Systems biology for organotypic cell cultures.

Translating in vitro biological data into actionable information related to human health holds the potential to improve disease treatment and risk assessment of chemical exposures. While genomics has identified regulatory pathways at the cellular level, translation to the organism level requires a multiscale approach accounting for intra-cellular regulation, inter-cellular interaction, and tiss...

Rapid Single-Cell Electroporation for Labeling Organotypic Cultures

Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in reasonable periods of time. A novel system that will rapidly load, clean, and accurately position ...

Organotypic hippocampal slice cultures.

The hippocampus, a component of the limbic system, plays important roles in long-term memory and spatial navigation. Hippocampal neurons can modify the strength of their connections after brief periods of strong activation. This phenomenon, known as long-term potentiation (LTP) can last for hours or days and has become the best candidate mechanism for learning and memory. In addition, the well ...

Organotypic vibrosections: Novel whole sagittal brain cultures

In vitro cell culture models are of enormous importance in neuroscience research and organotypic brain slices are found to be a potent model very close to the in vivo situation. Brain slices can be cultured as single slices or as co-slices. However, there is need to culture whole brain sections, containing the complex functional architecture. The aim of the present study was to develop and char...

Organotypic Cultures of the Rat Retina